Salt effects in the glutathione-facilitated reactivation of reduced bovine pancreatic ribonuclease.
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چکیده
منابع مشابه
Salt effects in the glutathione-facilitated reactivation of reduced bovine pancreatic ribonuclease.
The rate of regeneration of reduced RNase by glutathione was examined in the presence of several added substances: substrate, phospholipid, other proteins, bacterial ribosomes, and neutral salts. Of these, only neutral salts showed substantial effects. K2HOP4 and (NH4)2SO4 strongly accelerated regeneration, the alkali chlorides showed moderate acceleration or inhibition, while LiBr and KSCN str...
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With the glutathione system that leads to rapid regeneration of reduced lysozyme (Saxena, V. P., and Wetlaufer, D. B. (1971) Biochemistry 9, 5015), reduced pancreatic ribonuclease (RNase) regenerated activity in high yield (greater than 90%) but at a considerably lower rate (t1/2 approximately 75 min). Systematic examination of the effects upon regeneration of the concentrations and ratios of r...
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Recent studies of protein biosynt’hesis have indicated that the ribosome is the site at which amino acids are assembled into polypeptide chains (1). On the basis of experiments with rabbit reticulocytes and hen oviduct tissue, the rate of polypeptide chain growth appears to be approximately 1 residue per second (2, 3). Although much information is now available concerning the mechanism of this ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)40785-0